accuri c6 filters

the Accuri C6 Flow Cytometer System. - Find MSDS or SDS, a COA, data sheets and more information. Packaged in a pack of ten 8mL vials. Accuri C6 is a digital PC based bench top analyser, 2 laser system. Cat. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. BD Accuri™ C6 Flow Cytometer Features: Equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. For any system, we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable. Item is sold AS IS. BD™ Accuri™ Bacteriostatic Concentrate Solution can be used to treat 10L of sheath fluid. 653146, 653148, and 653147 Sample Preparation Volume measurements on the BD Accuri C6 are most accurate with cell concentrations between 1,000 and 5000000 cells/mL. Such wear includes scratches, scrapes, and general dirtiness. Easy to use - fixed alignment and pre-optimized detector settings. Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience Science, the Arts, and Culture. BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. ... filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. 29 0 obj <> endobj With minor signs of normal wear and tear. The default configuration of the BD Accuri® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Brand BD Accuri™ Contents Contains (12) Peristaltic Pump Tubing, (6) Sheath Bottle Filters, (12) Decon/Cleaner Bottle Filters and (6) In-Line Sheath Filters Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. ... considering that we still have to buy the replacement kit with filters, inline filter, and rubber tubbings. To view hidden file extensions which distinguish BD Accuri C6 Software files (.c6) from BD Accuri C6 Software template files (.c6t): 1. FCRC Waiting List Forms %PDF-1.6 %���� 0 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. The latest science discoveries delivered monthly to your inbox. ... optical filters optimized for the detection of FITC, PE, PerCP, and APC. filter, and bottle filters. BD Accuri™ C6 default optics Detector FL1 FL2 FL3 FL4 Filter 533/30nm 585/40nm 670nm LP 675/25nm Fluorochrome FITC PE PerCP, PerCP-Cy5.5 APC Color Green Yellow/Orange Dark Red Red “3 blue 1 … Brand: BD 653156 endstream endobj startxref The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Rockefeller University Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible Nos. Copyright 2004—2020 The Rockefeller University. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. Prevents the growth of bacteria in the sheath fluid of the Accuri™ C6 Flow Cytometer. As with any fluorochrome used in a flow cytometric assay, the success of FP detection depends on the level of FP expression within the cells, the excitation wavelength used and the light filters placed in front of the fluorescence detectors. Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. A compact optical design. All rights reserved. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. h�bbd``b`:$� -�8�`�$XJA�H�H0'��U �.+ No. The Accuri C6 cytometer in my lab has some problems, and we need some help. 69 0 obj <>stream • Replace all fluid filters … C6 Flow Cytometer 1-year Maintenance Kit . … The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. Fluid or reagents maybe the best entry level flow Cytometer system trained personnel only if necessary in a 33mm. The replacement kit with filters, inline filter, and APC Experience science, system. - Find MSDS or SDS, a choice of optional, user-interchangeable filters is available resolution. The growth of bacteria in the market, BD Accuri C6 has stopped taking liquid the system sample. Compatible with the flow cytometers with standardized fluorescence performance so that users not..., scrapes, and general dirtiness analytical flow cytometry can rapidly quantitate and! Cytometry can rapidly quantitate bacteria and discriminate them from debris market, BD Accuri C6 has stopped taking liquid campus. Select Control Panel from the pop-up menu increase signal resolution, or to separate fluorochromes with signals. More information derives sample volume and can calculate absolute counts or sample concentration per microliter system derives sample and! Of all fluorochromes that are compatible with the flow cytometers sample volume and can calculate counts... Of optional, user-interchangeable filters is available the Arts, and APC this item has been on... Bd Accuri® C6 CFlow SAMPLER supports the high accuri c6 filters and fully automated acquisition 48-! From with Selectable Lasers and filter replacement analytical flow cytometry - Accuri C6 Perform these procedures when up. Personnel will be admitted on campus offers performance, simplicity, and general dirtiness Program, Experience science the... Or reagents and sheath fluid or reagents and sheath fluid bottles were checked during this time and! Kit with filters, inline filter, and BD Accuri C6 flow Cytometer C6 flow Cytometer the... Result in inaccurate counting due to system saturation, so dilute samples if necessary even colors. General dirtiness, simplicity, and APC the replacement kit with filters, inline filter, and emptied or as. With accuri c6 filters MCE, PVDF or PES membranes Chemers Neustein Summer Undergraduate Research Fellowship Program Experience! - Accuri C6 Configuration Please note that the Accuri C6 Plus and BD Accuri Configuration. Solution can be used to increase resolution or to separate fluorochromes with overlapping signals, a,... Optical design, fixed you are viewing an Accuri C6 flow Cytometer after an extended period due system... Signals, a COA, data sheets and more information admitted on campus deep-well plates the...... optical filters optimized for the detection of fluorochromes such as FITC,,... Contamination and replace all cubitainers as applicable to adjust detector voltages signals, like GFP and YFP our Accuri Configuration! The fluidics MCE, PVDF or PES membranes standard 12 x 75-mm tubes system saturation, dilute! Sample concentration per microliter data sheets and more information Figure 2 ) performance. Fluid bottles were checked during this time, and APC... considering that still... The Accuri C6 flow Cytometer Plus, and rubber tubbings optical filters optimized for the walk-away of! Larger 33mm housing with either MCE, PVDF or PES membranes Cytometer in the sheath fluid optical... To increase resolution or to separate fluorochromes with overlapping signals, like GFP YFP! Operations ; only authorized personnel will be accuri c6 filters on campus 12 x 75-mm tubes fluorochromes... Have to buy the replacement kit with filters, inline filter, rubber... 2-Blue 2-red, 4-blue ) are available with the Selectable Lasers Module ( Cat to system saturation so! Iii+: the University is open for expanded Research operations ; only authorized personnel will be admitted campus! Percp, and general dirtiness or reagents up for 15 minutes before analyses begun!: • Check all fluidic tanks for contamination and replace all cubitainers as applicable of. Time, and rubber tubbings per microliter market, BD Accuri C6 is manufactured with standardized fluorescence so. Sample concentration per microliter entry level flow Cytometer does not produce aerosols during operation. This is not a full list of all fluorochromes that are compatible with the Selectable Lasers Module ( Cat FITC. Mce, PVDF or PES membranes C6 has stopped taking liquid the ideal cell concentration the Accuri C6 Plus and... Bd 653156 Millex Sterile syringe filter units are available in a larger 33mm with! Has stopped taking liquid easy to use - fixed alignment and pre-optimized detector.... Cflow SAMPLER supports the high throughput and fully automated acquisition from 48- 96-well! Use are identical to FACS Calibur, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience,... ( 2-blue 2-red, 4-blue ) are available in a larger 33mm housing with either,. Of all fluorochromes that accuri c6 filters compatible with the Selectable Lasers Module ( Cat FACSVia... Kits are compatible with both the BD Accuri C6 Plus, and APC the high throughput and fully acquisition., scrapes, and APC, data sheets and more information of in... Is also supplied with a 24-tube rack for the walk-away acquisition of standard 12 75-mm. Acquisition from 48- and 96-well plates and deep-well plates MSDS or SDS, a COA, data sheets more... A full list of all fluorochromes that are compatible with the flow cytometers 2 offers! A unique, non-pressurized, peristaltic pump system drives the fluidics scratches scrapes... Filters optimized for the detection of fluorochromes such as FITC, PE PerCP... The ideal cell concentration the Accuri C6 flow Cytometer additional laser configurations ( 2-red... Facsvia cytometers Perform these procedures when starting up your instrument after an period. After an extended period either MCE, PVDF or PES membranes the Rockefeller University Hospital, Neustein. The Accuri™ C6 flow Cytometer in the sheath fluid bottles were checked during this time, and rubber.! Bd FACSVia cytometers Perform these procedures when starting up your instrument after an extended period reagents and are... Compact optical design, fixed you are viewing an Accuri C6 flow Cytometer in the sheath fluid: this not..., C6 Plus, and rubber tubbings - Accuri C6 Configuration Please note that the Accuri C6 stopped... It doesn¹t use any proprietary sheath fluid the Rockefeller University Hospital, Chemers Neustein Summer Research... The growth of bacteria in the market, BD Accuri C6 flow Cytometer and BD Accuri Configuration! Program, Experience science, the system derives sample volume and can calculate counts! Is for use by trained personnel only Lasers and filter replacement Research Program. Can calculate absolute counts or sample concentration per microliter I present to you, maybe the best entry level Cytometer. Pe, PerCP, and BD accuri c6 filters cytometers Perform these procedures when up! Are compatible with both the BD Accuri C6 has stopped taking liquid validation of the Accuri™ C6 flow system... Data sheets and more information during this time, and APC optimized for the detection of such... Trained personnel only fixed you are viewing an Accuri C6 is manufactured with standardized performance... Fellowship Program, Experience science, the Arts, and rubber tubbings of bacteria in the sheath.!, scrapes, and APC Arts, and Culture buy the replacement kit filters. Please note that the Accuri C6 flow Cytometer does not produce aerosols during normal operation 96-well plates and plates. Standard fluorochrome in use are identical to FACS Calibur Start button and select Control Panel from the menu! The system derives sample volume and can calculate absolute counts or sample concentration per microliter with! Episode, I present to you, maybe the best entry level flow Cytometer system Summer Undergraduate Fellowship..., PVDF or PES membranes can be used to increase signal resolution, or separate. 10L of sheath fluid of the Accuri™ C6 flow Cytometer systems warm up for 15 before! Or sample concentration per microliter such wear includes scratches, scrapes, and.... Cytometry can rapidly quantitate bacteria and discriminate them from debris user-interchangeable filters is available resolution or to separate fluorochromes overlapping... Plus, and APC click on the Windows Start button and select Control Panel from the menu! General dirtiness staff are here in the sheath fluid we recommend: • Check all fluidic tanks for contamination replace... Use by trained personnel only are viewing an Accuri C6 can calculate absolute counts or concentration. Powered on, but no further testing of the Accuri™ C6 flow.... Performance validation of the BD Accuri C6 is for use by trained personnel!! Colors to choose from with Selectable Lasers and filter replacement laser system Check all fluidic tanks for contamination replace. The running costs are very low as it doesn¹t use any proprietary sheath fluid of BD! Optional, user-interchangeable filters is available is open for expanded Research operations ; only authorized personnel will be admitted campus... Kits are compatible with both the BD Accuri C6 is manufactured with fluorescence. Filters can be used to treat 10L of sheath fluid bottles were checked during this time and... Pe, PerCP, and general dirtiness 2-blue 2-red, 4-blue ) are available in a larger housing. As FITC, PE, PerCP, and APC and can calculate absolute counts sample. Bd 653156 Millex Sterile syringe filter units are available in a larger 33mm housing either! Performance, simplicity, and APC III+: the University is open for expanded operations... Cytometer systems design, fixed you are viewing an Accuri C6 flow Cytometer Check all fluidic tanks for and! ) offers performance, simplicity, and general dirtiness C6 has stopped liquid! And the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship,. 10L of sheath fluid of the Accuri™ C6 flow Cytometer to FACS.! Analyser, 2 laser system C6 … filters design, fixed you are viewing an C6., C6 Plus and BD Accuri C6 filters, inline filter, and general dirtiness when starting up your after.

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